Using data from GTM, NIW, HEE, and PDB reserves.

Equation 1 (Watras et al. 2017): \(F_{r} = \frac{F_{m}}{1 + \rho(T_{m} - T_{r})}\)

Temperature quenching of chlorophyll fluorescence (A) in water from Pellicer Creek, Florida by the GTMNERR, (B) Winyah Bay, South Carolina by the NIWNERR, (C) Kane'ohe Bay, Hawaii by HEENERR, and (D) Padilla Bay, Washington by PDBNERR. In panels (E,F,G, and H) the temperature quench was removed by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

Temperature quenching of chlorophyll fluorescence (A) in water from Pellicer Creek, Florida by the GTMNERR, (B) Winyah Bay, South Carolina by the NIWNERR, (C) Kane’ohe Bay, Hawaii by HEENERR, and (D) Padilla Bay, Washington by PDBNERR. In panels (E,F,G, and H) the temperature quench was removed by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

GTMNERR

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Pellicer Creek, Florida. The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Pellicer Creek, Florida. The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

General comments:

  • Slight jump in non-diluted water
  • Non-linear at higher temps
  • Slopes at different concentrations don’t follow Watras pattern
  • Slope in blank - could indicate sensor problem rather than, or in addition to, quenching
  • Applying Watras equation correction didn’t seem to work
  • Re-run experiment?
  • May need to use mixed effects model (Watras equation 4) to account for the effects of temperature on both the sensor and the energy decay pathways

NIWNERR

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Winyah Bay, South Carolina The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Winyah Bay, South Carolina The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

HEENERR

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Kane'ohe Bay, Hawaii. The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Kane’ohe Bay, Hawaii. The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

PDBNERR

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Padilla Bay, Washington. The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

Temperature quenching of chlorophyll (A) fluorescence in unfiltered water from Padilla Bay, Washington. The temperature quench was removed (B) by adjusting the raw data to a reference temperature of 20 (C) using Eq. 1.

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. And was created by Shannon Dunnigan | Shannon.Dunnigan@FloridaDEP.gov | skdunnigan